A study of the effects of mutagenesis of the promoter elements of P1-RFP recombinant plasmid on subsequent RFP expression. This study examined single-nucleotide substitutions mutations of the -10 region, -35 region, and Ribosomal Binding Site (RBS) of the P1 promoter. There was a 3 page maximum for the content in the report, excluding references. One page of supplemental information was allowed.